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7:54
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Amoeba Sisters
PCR (Polymerase Chain Reaction)
Join The Amoeba Sisters as they explain the biotechnology technique PCR. This video goes into the basics of how PCR works as well as two examples of its potential use. Table of Contents: 00:00 Intro 1:34 How does PCR work? 4:31 Why use PCR? 5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19 ...
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En una PCR, los primers son pequeños fragmentos de DNA que funcionan como puntos de inicio para que la enzima copie una región específica del material genético. En otras palabras, le dicen a la reacción exactamente qué segmento quieres amplificar. Diseñarlos bien es clave, porque si haces unos primers “chafas”, la reacción puede amplificar cosas que no quieres, no amplificar nada, o generar resultados confusos. Un buen diseño considera aspectos como la longitud, la temperatura de alineamiento, e
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